CIL:40687, Mus musculus, fibroblast. CIL. Dataset

NCBI Organism:Mus musculus; Cell Types:fibroblast Cell Components:nuclear chromosome Biological process:chromosome organization, mitosis, mitotic metaphase;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40687^* Cite

Description

High voltage (1MeV) transmission electron microscopy image of an isolated metaphase chromatid pair from a mouse A9 fibroblast, showing the fiber-like structure.

Technical Details

Cells in metaphase were exposed to 75 mM KCl, spread on water, picked up on grids, stained with uranyl acetate and critical point dried. See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: fibroblast
Cell Line: A9
Cellular Component: nuclear chromosome

Biological Context

Biological Process: chromosome organization; mitosis; mitotic metaphase

Attribution

Names: Hans Ris

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40687
Archival Resource Key (ARK): ark:/b7295/w9cil40687

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: chemically fixed tissue; critical_point dried specimen
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4025px	2.5nm
Y	5244px	2.5nm