CIL:40683, Mus musculus, fibroblast. CIL. Dataset

NCBI Organism:Mus musculus; Cell Types:fibroblast Cell Components:nuclear chromosome Biological process:chromosome organization, mitosis, mitotic metaphase;

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40683^* Cite

Description

High voltage (1MeV) transmission electron microscopy image of an isolated metaphase chromatid pair from a mouse A9 cultured cell, showing the fiber-like underlying structure. The image was taken with a specimen tilt of 46 degrees. Grouped with it is an image with a tilt of 53 degrees, providing a pair that affords an oblique stereo view of the chromosome.

Technical Details

Chromosomes were isolated from mouse A9 cells arrested in metaphase using a non-aqueous protocol, placed on a grid, and stained with uranyl acetate. See also: H. Ris 1981 Steroscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: fibroblast
Cell Line: A9
Cellular Component: nuclear chromosome

Biological Context

Biological Process: chromosome organization; mitosis; mitotic metaphase

Attribution

Names: Hans Ris

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40683
Archival Resource Key (ARK): ark:/b7295/w9cil40683

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; critical_point dried specimen
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4110px	1.27nm
Y	6674px	1.27nm