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NCBI Organism:Drosophila melanogaster; Cell Components:microtubule, cytoskeleton; Biological process:microtubule cytoskeleton organization, ;
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40658*  Cite 
Description

Time series spinning disc confocal images of Drosophila S2 cells stably expressing pMT-Eos2-alpha tubulin. A diagonal ~2.5 micrometer strip was photoconverted and images taken every minute for 20 minutes. Microtubules clearly migrate beyond the confines of the photoconverted strip. This image stack is part of a grouped set that includes a corresponding time lapse movie.

Technical Details

Time series were recorded using a Nikon U-2000 inverted microscope equipped with a Nikon 100x NA 1.4 objective lens, Perfect Focus System, CSU-10 spinning disc confocal head (Yokogawa) and Evolve EMCCD camera (Roper Scientific) driven by Nikon Elements software. See also: A.L. Jolly et al. 2010 Proc Natl Acad Sci USA 107:12151-12156.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Line
S2
Cellular Component
microtubule
cytoskeleton
Biological Context
Biological Process
microtubule cytoskeleton organization
microtubule
Attribution
Names
Vladimir Gelfand
Urko del Castillo
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40658
Archival Resource Key (ARK)
ark:/b7295/w9cil40658
Grouping This image is part of a group.
Imaging
Image Type
electron multiplying CCD (EMCCD)
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
mEOS2FP
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 411px ——
Y 456px ——
Time 60 seconds 20