CIL:40609, Saccharomyces cerevisiae. CIL. Dataset

NCBI Organism:Saccharomyces cerevisiae; Cell Components:mitochondrion, vacuole, ribosome; Biological process:organelle organization

Licensing

This image is copyright protected. Any public or private use of this image is subject to prevailing copyright laws. Please contact the content provider of this image for permission requests.

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40609^* Cite

Description

A 5 nm tomographic slice from a vitreous section of a Saccharomyces cerevisiae cell. (M) is a Mitochondrion and (V) a vacuole. Scale bar, 100 nm. Upper Panel: A high-resolution density map of the averaged 80S ribosome constructed using the ribosomes seen in the background image. Lower Panel: A select area from the 80S ribosome Macromolecular Atlas displaying a putative polyribosome cluster

Technical Details

Image collected on a FEI Titan Krios.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cellular Component: mitochondrion; vacuole; ribosome

Biological Context

Biological Process: organelle organization

Attribution

Names: Jason Pierson; Jose Jesus Fernandez; Matthijn Vos; Jose L. Carrascosa; Peter J. Peters
Link: FEI Life Science Images
OTHER: Netherlands Cancer Institute; Centro Nacional de Biotecnologia-Consejo Superior de Investigaciones Cientificas

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40609
Archival Resource Key (ARK): ark:/b7295/w9cil40609

Imaging

Image Type: recorded image; computer graphic
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: detection of electrons
Source of Contrast: differences in adsorption or binding of stain
Processing History: surface rendering

Sample Preparation

Methods: chemically fixed tissue
Relation To Intact Cell: sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	670px	——
Y	583px	——