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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40565*  Cite 
Description

The tubules arising from late DV-III expand at their distal ends into rounded shapes. These expanded ends contain a glycocalyx like the secondary lysosomes and large IMPs also like secondary lysosomes. TEM taken on 8/7/80 by R. Allen with Hitachi HU11A TEM. Neg. 12,250X. Part published in J. Cell Biol. 99:1955-1959, 1984. Adapted with permission.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
phagolysosome
Biological Context
Biological Process
digestive system process
Attribution
Names
Richard Allen (University of Hawaii)
Published
J Cell Biol. 1984 Dec;99(6):1955-9.
Pubmed
6501410
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40565
Archival Resource Key (ARK)
ark:/b7295/w9cil40565
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 4596px 1.2nm
Y 5148px 1.2nm