CIL:40319, Homo sapiens, epithelial cell, cervical carcinoma. CIL. Dataset

NCBI Organism:Homo sapiens; Cell Types:epithelial cell, ; Cell Components:nuclear pore, nuclear envelope; Biological process:nucleus organization, nuclear transport, nuclear envelope organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40319^* Cite

Description

Transmission electron micrograph of a thin section of the nucleus of a HeLa cell with mutant ALADIN. This grazing section of the nucleus shows en face views of nuclear pores which appear to have a normal ultrastructure. From a study of the role of the nuclear porin ALADIN. Other images in this group show further details of nuclear pore structure in normal and mutant cells.

Technical Details

Normal HeLa cells expressing were fixed with glutaraldehyde, embedded in epoxy resin, thin sectioned, and imaged using a Philips CM10 TEM. See: J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: epithelial cell; cervical carcinoma
Cell Line: HeLa
Cellular Component: nuclear pore; nuclear envelope

Biological Context

Biological Process: nucleus organization; nuclear transport; nuclear envelope organization

Attribution

Names: Janet Cronshaw; Michael Matunis
Published: J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827
Pubmed: 12730363
Link: Proceedings National Academy of Sciences USA

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40319
Archival Resource Key (ARK): ark:/b7295/w9cil40319

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: osmium tetroxide; uranyl salt; lead salt
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	140px	——
Y	133px	——