CIL:40316, Homo sapiens, epithelial cell, cervical carcinoma. CIL. Dataset

NCBI Organism:Homo sapiens; Cell Types:epithelial cell, ; Cell Components:nucleus, nuclear envelope, nuclear chromatin, nucleolus; Biological process:nucleus organization, nuclear transport;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40316^* Cite

Description

Transmission electron micrograph of a thin section of the nucleus of a normal HeLa cell - from a study of the role of the nuclear porin ALADIN. Other images in this group show details of nuclear pore structure in cross section and tangential section.

Technical Details

Normal HeLa cells expressing were fixed with glutaraldehyde, embedded in epoxy resin, thin sectioned, and imaged using TEM. See: J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: epithelial cell; cervical carcinoma
Cell Line: HeLa
Cellular Component: nucleus; nuclear envelope; nuclear chromatin; nucleolus

Biological Context

Biological Process: nucleus organization; nuclear transport

Attribution

Names: Janet Cronshaw; Michael Matunis
Published: J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827
Pubmed: 12730363
Link: Proceedings National Academy of Sciences USA

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40316
Archival Resource Key (ARK): ark:/b7295/w9cil40316

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: osmium tetroxide; uranyl salt; lead salt
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	226px	——
Y	307px	——