CIL:40269, Sus scrofa domestica, kidney epithelial cell. CIL. Dataset

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40269^* Cite

Description

Time lapse movie of a porcine kidney LLCPK1 cell stably expressing EGFP-alpha tubulin. The movie shows microtubule behavior from early prophase through metaphase.

Technical Details

Cells were imaged using a Nikon Eclipse TE300 microscope with 100x phase NA 1.4 objective lens and spinning disk confocal head (Perkin Elmer) and images captured with a MicroMax cooled CCD camera. Images were captured every 2 sec and are displayed at 7.5 frames/sec. See also:N Rusan and P Wadsworth 2005. Centrosome fragments and microtubules are transported asymmetrically away from division plane in mitosis.J Cell Biol 168:21-28.

Biological Sources

NCBI Organism Classification: Sus scrofa domestica
Cell Type: kidney epithelial cell
Cell Line: LLC-PK1
Cellular Component: microtubule cytoskeleton

Biological Context

Biological Process: mitosis; mitotic metaphase; microtubule cytoskeleton organization

Attribution

Names: Nasser Rusan; Patricia Wadsworth

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40269
Archival Resource Key (ARK): ark:/b7295/w9cil40269

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD)
Image Mode: spinning disk confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Data Qualifiers: processed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	510px	——
Y	423px	——

Time	2 seconds