CIL:40229, Mus musculus, Purkinje cell. CIL. Dataset

NCBI Organism:Mus musculus; Cell Types:Purkinje cell Cell Components:dendrite, neuronal cell body; Biological process:cell projection organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40229^* Cite

Description

A Purkinje neuron from the cerebellum of a 2 month old male C57BL/6 mouse, injected with Lucifer Yellow. The elaborate, highly branched dendritic arbor is revealed in this maximum projection image which was generated by compiling a z-series of optical sections. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Tissue was perfused with paraformaldehyde, sectioned (100 µm) on a vibratome and mounted in gelvatol, and imaged on a Biorad Radiance 2000 Confocal using a 60x 1.4NA objective.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: Purkinje cell
Cellular Component: dendrite; neuronal cell body

Biological Context

Biological Process: cell projection organization

Attribution

Names: Maryann Martone; Andrea Price; Andrea Thor; Hiro Hakozaki; Masako Terada
Link: CCDB 7

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40229
Archival Resource Key (ARK): ark:/b7295/w9cil40229

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: intracellular filling
Visualization Methods: lucifer yellow
Data Qualifiers: processed data

Sample Preparation

Relation To Intact Cell: vibratome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	——
Y	533px	——