CIL:40165, Mus musculus, peripheral neuron, myelinating Schwann cell. CIL. Dataset

NCBI Organism:Mus musculus; Cell Types:peripheral neuron, myelinating Schwann cell; Cell Components:node of Ranvier, myelin sheath; Biological process:regulation of action potential in neuron

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40165^* Cite

Description

Single slice through a single tilt tomogram of the Node of Ranvier from mouse sciatic nerve prepared by high pressure freezing and freeze substitution of aldehyde fixed material. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information see: Sosinsky et al. (2005) Development of a model for microphysiological simulations: small nodes of ranvier from peripheral nerves of mice reconstructed by electron tomography. Neuroinformatics. 3(2):133-62. PMID: 15988042

Technical Details

Tissue was obtained from a postnatal day 3 male mouse. Images were obtained using a JEOL 4000 microscope. Single tilt tomogram spanned -60 to 60° at 2° increments. Magnification: 25000X, accelerating voltage: 400.0 KeV.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: peripheral neuron; myelinating Schwann cell
Cellular Component: node of Ranvier; myelin sheath

Biological Context

Biological Process: regulation of action potential in neuron

Attribution

Names: Mark Ellisman; Gina Sosinsky
Link: CCDB 49

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40165
Archival Resource Key (ARK): ark:/b7295/w9cil40165

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode
Parameters Imaged: electron density
Data Qualifiers: processed data

Sample Preparation

Methods: crosslinking-fixative fixed tissue; FBbi:00000621
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	950px	——
Y	950px	——