CIL:40018, Mus musculus, CNS neuron (sensu Vertebrata). CIL. Dataset

NCBI Organism:Mus musculus; Cell Types:CNS neuron (sensu Vertebrata) Cell Components:neuronal cell body, neuron projection, dendrite;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40018^* Cite

Description

Summed Z projection through a through-focus series of a medium spiny neuron from the neostriatum of a dopamine-transporter knock out mouse, filled with Lucifer Yellow and then photooxidized. The neuron is not completely contained within the section so part of it is missing. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database, where the reconstruction of the entire cell can also be found.

Technical Details

Tissue was prepared from a 6 month adult male mouse strain, C57BL/129SvJ with a dopamine transporter knock-ou. Tissue sections, 100µm, were generated using a vibratome. The entire through series of images were gathered using a Bio-Rad 1024 confocal with a Zeiss Plan Apochromat 63X 1.4 NA.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: CNS neuron (sensu Vertebrata)
Cellular Component: neuronal cell body; neuron projection; dendrite

Attribution

Names: Diana Price; Aki Laakso; Maryann Martone; Naoko Yamada; Andrea Thor; Monica Berlanga; Michele Cyr
Link: CCDB 3659

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40018
Archival Resource Key (ARK): ark:/b7295/w9cil40018

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: confocal microscopy
Visualization Methods: intracellular filling
Data Qualifiers: processed data

Sample Preparation

Relation To Intact Cell: vibratome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	——
Y	512px	——