CIL:39960, Rattus norvegicus, Purkinje cell, astrocyte, cerebellar granule cell. CIL. Dataset

NCBI Organism:Rattus norvegicus; Cell Types:Purkinje cell, astrocyte, cerebellar granule cell; Cell Components:cell projection cytoplasm, cell body, nucleus, neuronal cell body; Biological process:cerebellum structural organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39960^* Cite

Description

High resolution montage of a sagittal section through the cerebellar cortex of an adult male rat (80g), triple-labeled to reveal nuclei (Hoescht stain, blue), Purkinje neurons (IP3 receptor, green) and astroglia (glial fibrillary acidic protein, GFAP, red). This image is a maximum projection of the Z-stack through the tissue, and has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

80µm thick sections were mounted in gelvitol using coverslips with a 1.0 µm thickness. Images were acquired with a BioRad RTS 2000MP Multiphoton microscope using a 40X 1.3NA oil immersion objective. This mosaic image was reconstructed with 10% overlap of individual images; image normalization was performed using ImageJ.

Biological Sources

NCBI Organism Classification: Rattus norvegicus
Cell Type: Purkinje cell; astrocyte; cerebellar granule cell
Cellular Component: cell projection cytoplasm; cell body; nucleus; neuronal cell body

Biological Context

Biological Process: cerebellum structural organization

Attribution

Names: Tom Deerinck
Link: CCDB 23

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39960
Archival Resource Key (ARK): ark:/b7295/w9cil39960

Imaging

Image Type: recorded image
Image Mode: multi-photon microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope; differences in adsorption or binding of stain
Visualization Methods: primary antibody plus labeled secondary antibody; Hoechst 33342
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: vibratome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	0.36µm
Y	478px	0.36µm