CIL:39956, Rattus norvegicus, Purkinje cell. CIL. Dataset

NCBI Organism:Rattus norvegicus; Cell Types:Purkinje cell Cell Components:dendrite, neuronal cell body; Biological process:cell projection organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39956^* Cite

Description

A Purkinje neuron from the cerebellum of an adult rat, injected with Lucifer Yellow. The elaborate, highly branched dendritic arbor is revealed in this maximum projection image which was generated by compiling a z-series of optical sections. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Image was acquired with a BioRad MRC 1024 Confocal using a 40X NA 1.3 oil immersion objective. A manual tracing of the complete dendritic arbor was done using Neurolucida 4.35c software, and can be viewed at the CCDB link provided.

Biological Sources

NCBI Organism Classification: Rattus norvegicus
Cell Type: Purkinje cell
Cellular Component: dendrite; neuronal cell body

Biological Context

Biological Process: cell projection organization

Attribution

Names: Maryann Martone; Eric Bushong; Ed Esquenazi
Link: CCDB 2

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39956
Archival Resource Key (ARK): ark:/b7295/w9cil39956

Imaging

Image Type: recorded image
Image Mode: confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: intracellular filling
Visualization Methods: lucifer yellow
Data Qualifiers: processed data

Sample Preparation

Relation To Intact Cell: vibratome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	502px	——
Y	650px	——