Images of negatively stained chromatin fibers prepared from tandem arrays of a nucleosome positioning sequence reconstituted with purified histones together with the results of Monte Carlo simulations of chromatin folding. Top left - 167 bp repeat with no linker histone; upper right - 197 bp with without linker histone; lower left 167 bp with linker histone H5; lower right 197 bp with linker histone. . See: Fig 4 in Routh et al. (2008) Nucleosome repeat length and linker histonen stoichiometry determine chromatin fiber structure. Proc Natl Acad Sci USA 105:8872-8877.
Reconstituted chromatin was fixed with 0.1% glutaraldehyde for 30 min, deposited on glow discharged carbon films, negatively stained with aqueous uranyl acetate, and examined with a FEI Tecnai F30 TEM operated at 200 KV. See also: Routh et al. (2008) Nucleosome repeat length and linker histonen stoichiometry determine chromatin fiber structure. Proc Natl Acad Sci USA 105:8872-8877.
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 960px | —— |
| Y | 720px | —— |
