CIL:39728. CIL. Dataset

Cell Components:, nucleosome; Biological process:DNA packaging, chromatin organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39728^* Cite

Description

TEM images of reconstituted chromatin prepared from 12-mer tandem repeats of a strong nuclear positioning sequence and purified histones. Samples were fixed with glutaraldehyde, applied to carbon films, and shadowed with Pt. (A, B) samples without linker histone H5 fixed in 5 mM NaCl; (C) Sample with H5, fixed in 150 mM NaCl; (D) sample with H5, fixed in 1 mM MgCl2. See Fig 1 in: S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322

Technical Details

Reconstitutes were fixed in the appropriate salt environment, applied to thin carbon films, air dried, and shadowed with Pt using electron bean evaporation (Balzers BA 080 vacuum evaporator. Grids were examined in a FEI Tecnai 12 TEM at 100KV with a LaB6 filament and tilted darkfield optics, and recorded using a TVIPS 2k CCD camera. See also: S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322.

Biological Sources

Cellular Component: chromatin; nucleosome

Biological Context

Biological Process: DNA packaging; chromatin organization

Attribution

Published: S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322
Pubmed: 19651606
Link: Proceedings National Academy of Sciences USA

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39728
Archival Resource Key (ARK): ark:/b7295/w9cil39728

Grouping This image is part of a group.

Imaging

Image Type: CCD
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	435px	——
Y	140px	——