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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39724*  Cite 
Description

Tetrahymena cells that are not forming a digestive vacuole often have a tube-like extension of the cytopharyngeal membrane attached to the deep fiber that extends several microns into the cytoplasm. The acidosome-like vesicles are docked at this membrane tube. TEM taken on 2/12/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 16,250X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Tetrahymena pyriformis
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cytostome
cytopharynx
acidosome
Biological Context
Biological Process
digestive system process
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39724
Archival Resource Key (ARK)
ark:/b7295/w9cil39724
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 4457px 0.92nm
Y 4974px 0.92nm