CIL:39645, Saccharomyces cerevisiae. CIL. Dataset

NCBI Organism:Saccharomyces cerevisiae; Cell Components:cell surface Biological process:cell cycle checkpoint, cell budding, regulation of cell budding, ;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39645^* Cite

Description

10-hour timelapse of the double mutant Clb2dbΔ, Clb5Δ, which has the destruction box region of the CLB2 gene, on the cell cycle control system in budding yeast. The yeast, Saccharomyces cerevisia, are growing in 2% raffinose, since this mutation is inviable in glucose. The images from two experiments done with Wild-type Saccharomyces cerevisiae as a control are available as CIL:39606-39625 and CIL:39646-39665. The images from two experiments done with the double mutant Clb2dbΔ, Clb5Δ are available as CIL:39626-39645 and CIL:39666-39685.

Technical Details

Images were collected at 4-minute intervals on a DeltaVision system equipped with a with a 60x PlanApo PH3 phase-contrast, oil immersion objective (NA = 1.4). This system has a motorized x-y-z stage, automatic focusing, motorized illumination shutters and a CoolSNAP_HQ2 camera.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cellular Component: cell surface

Biological Context

Biological Process: cell cycle checkpoint; cell budding; regulation of cell budding; Clb2dbΔ, Clb5Δ mutation

Attribution

Names: David Ball; Jean Peccoud
Published: Cell Cycle 10:6, 999-1009; March 15, 2011;

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39645
Archival Resource Key (ARK): ark:/b7295/w9cil39645

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: phase contrast microscopy
Parameters Imaged: elastic scattering of photons
Source of Contrast: differences in amount of elastic light scattering
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	0.213µm
Y	512px	0.213µm

Time	240 seconds	10 hr