CIL:39602, Saccharomyces cerevisiae. CIL. Dataset

NCBI Organism:Saccharomyces cerevisiae; Cell Components:, nucleus, cytoplasm; Biological process:regulation of cyclin-dependent protein kinase activity, G1/S transition of mitotic cell cycle;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39602^* Cite

Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cln1. Cln 1 is a cyclin-dependent protein kinase regulator. These phase and GFP z-series images are part of an image group containing CIL 39601-39605. Note that there are additional groups of images of other cell cycle regulator proteins by the same authors available in the Library.

Technical Details

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please read the microscopy section of the referenced paper for details before image analysis.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cellular Component: CLN1 protein; nucleus; cytoplasm

Biological Context

Biological Process: regulation of cyclin-dependent protein kinase activity; G1/S transition of mitotic cell cycle
Molecular Function: cyclin-dependent protein kinase regulator activity

Attribution

Names: David Ball; Jean Peccoud
Published: PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed: 22046265

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39602
Archival Resource Key (ARK): ark:/b7295/w9cil39602

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy; phase contrast microscopy
Parameters Imaged: fluorescence emission; elastic scattering of photons
Source of Contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization Methods: EGFP

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	213.4nm
Y	512px	213.4nm
Z	21px	0.5µm

Time	300 microns	61