CIL:39562, Saccharomyces cerevisiae. CIL. Dataset

NCBI Organism:Saccharomyces cerevisiae; Cell Components:, nuclear chromatin, nucleus; Biological process:arginine metabolic process, cell cycle, regulation of transcription, DNA-dependent, transcription, DNA-dependent, DNA-dependent DNA replication initiation, regulation of transcription from RNA polymerase II promoter;

Licensing

Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39562^* Cite

Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged MCM1. MCM1 is a transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes These phase, gfp z-series images are part of an image group that ranges from CIL:39561-39565. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

Technical Details

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cellular Component: MCM1 protein; nuclear chromatin; nucleus

Biological Context

Biological Process: arginine metabolic process; cell cycle; regulation of transcription, DNA-dependent; transcription, DNA-dependent; DNA-dependent DNA replication initiation; regulation of transcription from RNA polymerase II promoter
Molecular Function: DNA binding; DNA bending activity; DNA replication origin binding; sequence-specific DNA binding transcription factor activity

Attribution

Names: David Ball; Jean Peccoud
Published: PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed: 22046265

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39562
Archival Resource Key (ARK): ark:/b7295/w9cil39562

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy; phase contrast microscopy
Parameters Imaged: fluorescence emission; elastic scattering of photons
Source of Contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization Methods: EGFP; visualization of contiguous regions

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	213.4nm
Y	512px	213.4µm
Z	21px	0.5µm

Time	300 seconds	61