CIL:39556, Saccharomyces cerevisiae. CIL. Dataset

NCBI Organism:Saccharomyces cerevisiae; Cell Components:, spindle pole body, spindle, cellular bud neck; Biological process:G2/M transition of mitotic cell cycle, positive regulation of spindle pole body separation, regulation of cyclin-dependent protein kinase activity;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39556^* Cite

Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Clb2. Clb 2 is a cyclin-dependent protein kinase regulator, involving in G2/M transition of mitotic cell cycle and positively regulating spindle pole body separation. These phase and GFP z-series images are part of an image group containing CIL 39556-39560. Note that there are additional groups of images of other cell cycle regulator proteins by the same authors available in the Library.

Technical Details

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please read the microscopy section of the referenced paper for details before image analysis.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cellular Component: CLB2 protein; spindle pole body; spindle; cellular bud neck

Biological Context

Biological Process: G2/M transition of mitotic cell cycle; positive regulation of spindle pole body separation; regulation of cyclin-dependent protein kinase activity
Molecular Function: cyclin-dependent protein kinase regulator activity

Attribution

Names: David Ball; Jean Peccoud
Published: PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed: 22046265

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39556
Archival Resource Key (ARK): ark:/b7295/w9cil39556

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy; phase contrast microscopy
Parameters Imaged: fluorescence emission; elastic scattering of photons
Source of Contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization Methods: EGFP; visualization of contiguous regions
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	213.4nm
Y	512px	213.4nm
Z	21px	0.5µm

Time	300 microns	61