CIL:39552, Saccharomyces cerevisiae. CIL. Dataset

NCBI Organism:Saccharomyces cerevisiae; Cell Components:, nucleus, RENT complex, nucleolus; Biological process:chromatin silencing at rDNA, nucleolus organization, regulation of exit from mitosis, cell cycle, cell division, mitosis;

Licensing

Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39552^* Cite

Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged NET1. NET1 is a core subunit of the RENT complex that involves in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure. These phase, gfp images are part of an image group that ranges from CIL:39551-39555. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

Technical Details

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cellular Component: NET1 protein; nucleus; RENT complex; nucleolus

Biological Context

Biological Process: chromatin silencing at rDNA; nucleolus organization; regulation of exit from mitosis; cell cycle; cell division; mitosis
Molecular Function: rDNA binding

Attribution

Names: David Ball; Jean Peccoud
Published: PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed: 22046265

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39552
Archival Resource Key (ARK): ark:/b7295/w9cil39552

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy; phase contrast microscopy
Parameters Imaged: fluorescence emission; elastic scattering of photons
Source of Contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization Methods: EGFP; visualization of contiguous regions

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	213.4nm
Y	512px	213.4nm
Z	21px	0.5µm

Time	300 microns	61