CIL:39537, Saccharomyces cerevisiae. CIL. Dataset

NCBI Organism:Saccharomyces cerevisiae; Biological process:regulation of cell cycle, establishment of protein localization;

Licensing

Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39537^* Cite

Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Bck2, a Cln-independent activator of CLN1,2 expression. These phase and gfp z-stacks are part of image group that ranges from CIL:39536-39540. Note there are additional groups of images of other proteins involved in cell cycle regulation from the same authors available in the library.

Technical Details

The images were acquired using a 60x 1.42 NA objective lens on Delta Vision system equipped with CoolSNAP_HQ2 / HQ2-ICX285. Please see the microscopy section of the referenced paper for details prior to image analysis.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae
Cellular Component: BCK2 protein

Biological Context

Biological Process: regulation of cell cycle; establishment of protein localization

Attribution

Names: David Ball; Jean Peccoud
Published: PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26
Pubmed: 2204626

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39537
Archival Resource Key (ARK): ark:/b7295/w9cil39537

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy; phase contrast microscopy
Parameters Imaged: fluorescence emission; elastic scattering of photons
Source of Contrast: distribution of a specific protein; differences in amount of elastic light scattering
Visualization Methods: EGFP; visualization of contiguous regions
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	0.213µm
Y	512px	0.213µm
Z	21px	0.5µm