CIL:39516, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Vorticella convallaria; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:pellicle, rough endoplasmic reticulum, fibril; Biological process:cortical cytoskeleton organization, endoplasmic reticulum organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39516^* Cite

Description

Fibers that splay out from a mid piece pass to the pellicle. TEM taken on 4/5/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 10,250X. Published and adapted with permission from J. Cell Biol. 56:559-579, 1973. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Vorticella convallaria
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, # LB1690/1
Cellular Component: pellicle; rough endoplasmic reticulum; fibril

Biological Context

Biological Process: cortical cytoskeleton organization; endoplasmic reticulum organization

Attribution

Names: Richard Allen (University of Hawaii)
Published: J Cell Biol. 1973 Feb;56(2):559-79.
Pubmed: 4630196

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39516
Archival Resource Key (ARK): ark:/b7295/w9cil39516

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3696px	0.98nm
Y	4000px	0.98nm