CIL:39339, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Nassula; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:contractile vacuole pore, microtubule associated complex, cytoplasm; Biological process:, microtubule cytoskeleton organization, cytoplasm organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39339^* Cite

Description

Detail of CV pore. Microtubules encircle the pore wall and other microtubules radiate out from the pore toward the CV membrane. TEM taken on 3/27/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm.

Biological Sources

NCBI Organism Classification: Nassula
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1650/1
Cellular Component: contractile vacuole pore; microtubule associated complex; cytoplasm

Biological Context

Biological Process: contractile vacuole pore organization; microtubule cytoskeleton organization; cytoplasm organization

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39339
Archival Resource Key (ARK): ark:/b7295/w9cil39339

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4320px	1nm
Y	5955px	1nm