CIL:39323, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Vorticella convallaria; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:rough endoplasmic reticulum, lysosome, Golgi apparatus, cytoplasm, ; Biological process:Golgi organization, cytoplasm organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39323^* Cite

Description

Vorticella convallaria fixed for EM and buffered in cacodylate. The rough ER is not vesiculated as it is in collidine buffered fixatives but appears as sheets from which Golgi dictyosomes arise. A food vacuole is surrounded by primary lysosomes or cupulas. At the surface a continuous sheet of ER lies against the myonemes. TEM taken on 2/11/72 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Vorticella convallaria
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, # LB1690/1
Cellular Component: rough endoplasmic reticulum; lysosome; Golgi apparatus; cytoplasm; myoneme fiber

Biological Context

Biological Process: Golgi organization; cytoplasm organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39323
Archival Resource Key (ARK): ark:/b7295/w9cil39323

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4842px	0.77nm
Y	4576px	0.77nm