CIL:39319, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cell cortex, , trichocyst, cilium, cytoplasm; Biological process:cortical cytoskeleton organization, cytoplasm organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39319^* Cite

Description

The infraciliary lattice in this quick-freeze deep-etched image consists of a meshwork of branching fibers composed of many subfibers. Basal bodies and trichocyst tips extend through the openings of the mesh. Individual metal-coated fibrils of the mesh average 11 nm in diameter and are closely interlaced and cross-linked to other fibrils. TEM taken on 5/27/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cell cortex; mitochondria; trichocyst; cilium; cytoplasm

Biological Context

Biological Process: cortical cytoskeleton organization; cytoplasm organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39319
Archival Resource Key (ARK): ark:/b7295/w9cil39319

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	5498px	1.4nm
Y	6001px	1.4nm