CIL:39237, uncultured scuticociliate, Conchophthirus curtus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:uncultured scuticociliate, , ; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cell cortex, microtubule basal body, plasma membrane, cortical cytoskeleton, cortical microtubule cytoskeleton, mitochondrion, cilium axoneme, coated pit, oral apparatus; Biological process:cortical cytoskeleton organization, clathrin coat assembly, mitochondrion organization, microtubule basal body organization, oral apparatus organization;

Licensing

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Description

Vestibular kinities and the cortical architecture adjacent to the oral apparatus of Conchophthirus curtus. The negative magnification is 15,500X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. For greater detail see:Antipa, G. A. 1971. Structural differentiation in the somatic cortex of a ciliated protozoan, Conchophthirus curtus Engelmann, 1862. Protistologica 7:47l-501 and Antipa, G. A. and Small, E. B. 1971. A redescription of Conchophthirus curtus Engelmann, 1862 (Protozoa, Ciliatea). J. Protozool. 18:491-503.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.

Biological Sources

NCBI Organism Classification: uncultured scuticociliate; Conchophthirus curtus
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cell cortex; microtubule basal body; plasma membrane; cortical cytoskeleton; cortical microtubule cytoskeleton; mitochondrion; cilium axoneme; coated pit; oral apparatus

Biological Context

Biological Process: cortical cytoskeleton organization; clathrin coat assembly; mitochondrion organization; microtubule basal body organization; oral apparatus organization

Attribution

Names: Gregory Antipa (San Francisco State University)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39237
Archival Resource Key (ARK): ark:/b7295/w9cil39237

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	5124px	0.97nm
Y	4982px	0.97nm