CIL:39213, Homo sapiens, cardiomyocytes. CIL. Dataset

NCBI Organism:Homo sapiens; Cell Components:plasma membrane, nucleus, ; Biological process:, ;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39213^* Cite

Description

Fluorescent micrograph showing human induced pluripotent stem (iPS) cell-derived cardiomyocytes (CDI iCell) treated with 2 microM Erythromycin for 4 hours and stained for cell membrane permeabilization (Propidium Iodide, red), calcium mobilization (Fluo-4 AM, green) and Hoechst 33342 to visualize nuclei (blue). Images of live cells were taken with GE IN Cell Analyzer 1000 (20x objective).

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: cardiomyocytes
Cellular Component: plasma membrane; nucleus; calcium

Biological Context

Biological Process: erythromycin treatment; cell viablity assay

Attribution

Names: Karen Bernards; Phuong Nguyen; Yulia Ovechkina; Christine OâDay
OTHER: Ricerca Biosciences, Bothell, Washington, USA

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39213
Archival Resource Key (ARK): ark:/b7295/w9cil39213

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: Hoechst 33342; propidium iodide; Fluo-4 AM

Sample Preparation

Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1300px	——
Y	1028px	——