CIL:39205, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium caudatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:macronucleus, nuclear pore, nucleolus; Biological process:macronucleus organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39205^* Cite

Description

Nucleoli in a macronucleus of a cell undergoing micronuclear division. The nucleolus appears to have an organized surface layer composed of parallel threads of subunits. These may be developing ribosomal subunits that will soon be transported to the cytoplasm through the nuclear pores. TEM taken on 3/30/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 15,759X.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium caudatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: macronucleus; nuclear pore; nucleolus

Biological Context

Biological Process: macronucleus organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39205
Archival Resource Key (ARK): ark:/b7295/w9cil39205

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4572px	0.95nm
Y	5116px	0.95nm