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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39193*  Cite 
Description

Opercularia coarctata. Transmission electron micrograph of contracted cell body. This ciliate does not have rows of basal body/cilia over its whole surface but has cilia in the oral region and in a trochal band area. The pellicle is sculpted into ridges and grooves that circle the body. A layer of endoplasmic reticulum lies under the pellicle next to a layer of myonemal fibers. The macronucleus, contractile vacuole, mitochondria, and peristomeare evident in this view. TEM taken on 6/12/69 by R. Allen with Philips 300 operating at 60kV. Neg. 4,270X.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Opercularia [NCBITaxon:168247]
Opercularia coarctata
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, # LB1657/1
Cellular Component
macronucleus
oral apparatus
mitochondrion
cell cortex
cytoplasm
Attribution
Names
Richard Allen
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39193
Archival Resource Key (ARK)
ark:/b7295/w9cil39193
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 3756px 1.2nm
Y 4000px 1.2nm