CIL:39173, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Opercularia [NCBITaxon:168247], , ; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:macronucleus, oral apparatus, mitochondrion, cell cortex, cytoplasm, contractile vacuole, micronucleus; Biological process:macronucleus organization, cortical cytoskeleton organization, cytoplasm organization, contractile vacuole organization, micronucleus organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39173^* Cite

Description

This section is cut at a level where the macronucleus is folded so it appears to be in 2 parts and where the micronucleus is visible. Contractile vacuole, perioral kinetids, and the peristome is evident. TEM taken on 6/10/69 by R. Allen with Philips 300 operating at 60kV. Neg. 3,570X.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Opercularia [NCBITaxon:168247]; Opercularia coarctata
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, # LB1657/1
Cellular Component: macronucleus; oral apparatus; mitochondrion; cell cortex; cytoplasm; contractile vacuole; micronucleus

Biological Context

Biological Process: macronucleus organization; cortical cytoskeleton organization; cytoplasm organization; contractile vacuole organization; micronucleus organization

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39173
Archival Resource Key (ARK): ark:/b7295/w9cil39173

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3870px	1.4nm
Y	4000px	1.4nm