CIL:39158, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium caudatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:oral apparatus Biological process:oral apparatus organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39158^* Cite

Description

Cross section of the oral region (buccal cavity) posterior to the vestibulum. The cytopharynx is beginning on the dorsal surface and the ribbed wall, peniculi and quadrulus are present on the right and left. The floor of the buccal cavity is bordered by the filamentous reticulum and an anarchic field of basal bodies is found where the endoral membranelle terminates. TEM taken on 1/31/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 6,150X.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. There is a high resolution version of this image in the library (CIL:) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium caudatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: oral apparatus

Biological Context

Biological Process: oral apparatus organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39158
Archival Resource Key (ARK): ark:/b7295/w9cil39158

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3172px	1.3nm
Y	4000px	1.3nm