CIL:39156, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium caudatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:oral apparatus Biological process:oral apparatus organization

Licensing

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Description

Cross section through the more anterior part of the oral region showing the four more loosely spaced rows of cilia making up the quadrulus and more compact cilia of the peniculi on the left hand wall of the buccal cavity. The indentation of the endoral membranelle lies to the right just above the buccal overture. The vestibulum bearing somatic ciliature connects the buccal cavity with the outside of the cell. The ribbed wall covers the upper right side of the buccal cavity. mac, macronucleus. TEM taken on 1/31/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 5,850X.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium caudatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: oral apparatus

Biological Context

Biological Process: oral apparatus organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39156
Archival Resource Key (ARK): ark:/b7295/w9cil39156

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3901px	1.4nm
Y	4000px	1.4nm