CIL:39114, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Euplotes sp.; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:oral apparatus, cortical cytoskeleton; Biological process:oral apparatus organization, cortical cytoskeleton organization, cytoplasm organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39114^* Cite

Description

A view of the cytopharynx and its nascent food vacuole forming region. The flattened pharyngeal vesicles that may contain monolayers or bilayers of lipids seem to be equivalent to the discoidal vesicles of Paramecium that provide membrane for food vacuole growth. They approach and fuse with the cytopharyngeal membrane between the lamellae (cytopharyngeal ribbons). TEM taken on 7/29/67 by R. Allen with Philips 200 operating at 60kV. Neg. 9,000X.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Euplotes sp.
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Euplotes sp. from Carolina Biological Supply
Cellular Component: oral apparatus; cortical cytoskeleton

Biological Context

Biological Process: oral apparatus organization; cortical cytoskeleton organization; cytoplasm organization

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL39114
Archival Resource Key (ARK): ark:/b7295/w9cil39114

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3118px	1.1nm
Y	4000px	1.1nm