CIL:38961, glioblastoma. CIL. Dataset

Cell Components:nucleus, cytoplasm; Biological process:endocytosis

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38961^* Cite

Description

Glioblastoma cells in culture transfected with green fluorescent protein. The nuclei are stained blue with DAPI. The red spots are 0.02 micron fluorescent spheres that have been taken up into the cells by endocytosis. These cells have no endogenous P10 protein; P10 is tumour suppressor gene that is mutated in many different types of cancer. The absense of P10 protein is thought to increase cell mobility and possibly contribute to metastasis.

Technical Details

B0003675. Wellcome Images available under the following creative commons usage http://creativecommons.org/licenses/by-nc-nd/2.0/uk/

Biological Sources

Cell Type: glioblastoma
Cellular Component: nucleus; cytoplasm

Biological Context

Biological Process: endocytosis

Attribution

Names: Alex Gray
Link: Wellcome Images

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL38961
Archival Resource Key (ARK): ark:/b7295/w9cil38961

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP; 4',6-diamidino-2-phenylindole (DAPI)

Sample Preparation

Methods: chemically fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	550px	——
Y	576px	——