CIL:38901, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium caudatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cell cortex, cilium, , ; Biological process:cortical cytoskeleton organization, cytoplasm organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38901^* Cite

Description

Stacks of 4 or 5 kinetodesmal fibers occupy the longitudinal ridges in a cross-sectioned cell. The kds lie along the right side of each ridge (cell’s left side), and as the kds are tapered, the smallest cross sections lie closest to the ridge tip and these sections are also closest to the distal tips of the kds. Such a kd arose from basal bodies 4 or 5 cortical units posterior to the plane of the cross-section. Lateral ridges do not contain kds. TEM taken on 12/28/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 17,500X.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium caudatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cell cortex; cilium; kinetodesmal fiber; cytoplasm

Biological Context

Biological Process: cortical cytoskeleton organization; cytoplasm organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL38901
Archival Resource Key (ARK): ark:/b7295/w9cil38901

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4746px	0.86nm
Y	4936px	0.86nm