CIL:38895, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium caudatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cytoplasm, pellicle, plasma membrane, cell cortex; Biological process:plasma membrane organization, cytoplasm organization, organelle organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38895^* Cite

Description

A cell with a flat alveolar sac system. It is not clear why some cells have expanded alveoli and others have flattened alveoli. Cacodylate buffered fixative. TEM taken on 2/3/69 by R. Allen with Philips 300 operating at 60kV. Neg. 20,500X.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium caudatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: cytoplasm; pellicle; plasma membrane; cell cortex

Biological Context

Biological Process: plasma membrane organization; cytoplasm organization; organelle organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL38895
Archival Resource Key (ARK): ark:/b7295/w9cil38895

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3507px	0.98nm
Y	4150px	0.98nm