CIL:38871, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Euplotes sp.; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cytoplasm, macronucleus, food vacuole, contractile vacuole; Biological process:macronucleus organization, cytoplasm organization, ;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38871^* Cite

Description

In Euplotes a replication band of the macronucleus has larger heterochromatin masses on one side of the band after DNA replication has occurred. The replication band moves in the direction of lighter staining in the macronucleus. Vacuoles containing concretion bodies are observed. TEM taken on 8/03/67 by R. Allen with Philips 200 operating at 60kV. Neg. 7,200X. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Euplotes sp.
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Euplotes sp. from Carolina Biological Supply
Cellular Component: cytoplasm; macronucleus; food vacuole; contractile vacuole

Biological Context

Biological Process: macronucleus organization; cytoplasm organization; macronuclear replication

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL38871
Archival Resource Key (ARK): ark:/b7295/w9cil38871

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3359px	1.4nm
Y	4000px	1.4nm