CIL:38866, Colpoda cucullus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Colpoda cucullus; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cilium, microtubule basal body, cell cortex, cytoplasm, food vacuole; Biological process:cell motility, digestion;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38866^* Cite

Description

A high resolution image of a layer of mucocysts docked under the pellicle. The pellicle has a plasma membrane and an alveolar sac system. Both basal bodies of a dikinetid are ciliated, and the food vacuoles are plentiful and filled with bacteria. TEM taken on 6/5/69 by R. Allen with Philips 300 operating at 60kV. Neg. 4,270X. The raw film was scanned with an Epson Perfection V750 Pro. This high resolution image is suitable for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Colpoda cucullus
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England # LB1615/1
Cellular Component: cilium; microtubule basal body; cell cortex; cytoplasm; food vacuole

Biological Context

Biological Process: cell motility; digestion

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL38866
Archival Resource Key (ARK): ark:/b7295/w9cil38866

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2894px	1.2nm
Y	4000px	1.2nm