CIL:38721, Chlamydomonas reinhardtii. CIL. Dataset

NCBI Organism:Chlamydomonas reinhardtii; Cell Components:chloroplast, plastid, plastid thylakoid membrane; Biological process:photosynthesis, organelle organization;

Licensing

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Description

Isolated chloroplasts of the unicellular photosynthetic alga Chlamydomonas reinhardtii were cryofixed, freeze-fractured, and surface replicas observed by transmission electron microscopy. This close-up of wildtype thylakoids shows the particle size and distribution characteristic of 'B' fracture planes where the thylakoids are stacked. See Fig 5 in Goodenough and Staehelin 1971 J Cell Biol 48:595-619.

Technical Details

Isolated chloroplasts were cryoprotected with glycerol, cryofixed, fractured at -100C, shadowed with Pt, and replicas prepared by carbon coating. Cleaned replicas were examined with a Philips 300 TEM. See Goodenough and Staehelin 1971 J Cell Biol 48:595-619

Biological Sources

NCBI Organism Classification: Chlamydomonas reinhardtii
Cell Line: ac-5; ac-31
Cellular Component: chloroplast; plastid; plastid thylakoid membrane

Biological Context

Biological Process: photosynthesis; organelle organization

Attribution

Names: Ursula W. Goodenough; L. Andrew Staehelin
Published: UW Goodenough and LA Staehelin (1971) Structural differentiation of stacked and unstacked chloroplast membranes. J Cell Biol 48:595-619.
Pubmed: 4396088
Link: Journal of Cell Biology

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL38721
Archival Resource Key (ARK): ark:/b7295/w9cil38721

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating
Data Qualifiers: processed data

Sample Preparation

Methods: cryofixed tissue; freeze_fracture/freeze_etch
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	780px	——
Y	624px	——