CIL:38719, Chlamydomonas reinhardtii. CIL. Dataset

NCBI Organism:Chlamydomonas reinhardtii; Cell Components:chloroplast, plastid, plastid thylakoid membrane; Biological process:photosynthesis, organelle organization;

Licensing

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Description

Isolated chloroplast membranes of the unicellular photosynthetic alga Chlamydomonas reinhardtii were cryofixed, freeze-fractured, and surface replicas observed by transmission electron microscopy. These thylakoid membranes from wildtype cells include regions where the membrane fracture plane shows the particle size and distribution typical of the 'B' and 'C' surfaces of both stacked and unstacked membranes. See Fig 3 in Goodenough and Staehelin 1971 J Cell Biol 48:595-619.

Technical Details

Isolated thylakoid membranes were cryoprotected with glycerol, cryofixed, fractured at -100C, shadowed with Pt, and replicas prepared by carbon coating. Cleaned replicas were examined with a Philips 300 TEM. See Goodenough and Staehelin 1971 J Cell Biol 48:595-619.

Biological Sources

NCBI Organism Classification: Chlamydomonas reinhardtii
Cell Line: ac-5; ac-31
Cellular Component: chloroplast; plastid; plastid thylakoid membrane

Biological Context

Biological Process: photosynthesis; organelle organization

Attribution

Names: Ursula W. Goodenough; L. Andrew Staehelin
Published: UW Goodenough and LA Staehelin (1971) Structural differentiation of stacked and unstacked chloroplast membranes. J Cell Biol 48:595-619.
Pubmed: 4396088
Link: Journal of Cell Biology

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL38719
Archival Resource Key (ARK): ark:/b7295/w9cil38719

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating
Data Qualifiers: processed data

Sample Preparation

Methods: cryofixed tissue; freeze_fracture/freeze_etch
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1578px	——
Y	1590px	——