CIL:38715, Chlamydomonas reinhardtii. CIL. Dataset

NCBI Organism:Chlamydomonas reinhardtii; Cell Components:chloroplast, plastid, plastid thylakoid membrane; Biological process:photosynthesis, organelle organization;

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Description

Isolated chloroplast membranes of the unicellular photosynthetic alga Chlamydomonas reinhardtii were fixed, sectioned, and examined by transmission electron microscopy. Under the conditions used here, with the ac-31 mutant, thylakoids are completely unstacked. See Fig 19 in Goodenough and Staehelin 1971 J Cell Biol 48:595-619.

Technical Details

Isolated chloroplast membranes were fixed with glutaraldehyde, post fixed with osmium tetroxide, dehydrated, embedded in epoxy resin, and thin sections cut, stained and examined with a Hitachi HU-11C TEM.

Biological Sources

NCBI Organism Classification: Chlamydomonas reinhardtii
Cell Line: ac-5; ac-31
Cellular Component: chloroplast; plastid; plastid thylakoid membrane

Biological Context

Biological Process: photosynthesis; organelle organization

Attribution

Names: Ursula W. Goodenough; L. Andrew Staehelin
Published: UW Goodenough and LA Staehelin (1971) Structural differentiation of stacked and unstacked chloroplast membranes. J Cell Biol 48:595-619.
Pubmed: 4396088
Link: Journal of Cell Biology

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL38715
Archival Resource Key (ARK): ark:/b7295/w9cil38715

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: osmium tetroxide; uranyl salt; lead salt
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1374px	——
Y	1044px	——