CIL:37330, epidermal cell. CIL. Dataset

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37330^* Cite

Description

This video shows an epidermal cell loaded with a florescent lipid diOC-6. diOC-6 is a vital stain for the endoplasmic reticulum and mitochondria. In this sequence one sees the extension of ER tubules along microtubules into the middle part of the lamellipodium. These extensions of endoplasmic reticulum appear to be pushed back towards the cell body by the retrograde flows of actin in lamellipodia. Video made available by Mark Cooper thru Zebrafish - The Living Laboratory.

Biological Sources

Cell Type: epidermal cell
Cellular Component: endoplasmic reticulum; mitochondrion; lamellipodium

Biological Context

Biological Process: actin filament-based movement

Attribution

Names: Mark Cooper
Link: Fishscope
OTHER: Zebrafish-The Living Laboratory

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL37330
Archival Resource Key (ARK): ark:/b7295/w9cil37330

Imaging

Image Type: recorded image
Image Mode: differential interference contrast microscopy
Parameters Imaged: optical path length gradient
Source of Contrast: boundaries between regions with different refractive index
Visualization Methods: visualization of contiguous regions; DiOC6 (3,3'-dihexyloxacarbocyanine iodide)
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	320px	——
Y	240px	——