Transmission electron micrograph of thin section of rat liver with prominent Golgi and smooth ER associated vesicles. At lower right, the junction between hepatocytes harbors a bile canaliculus. The image illustrates the ethanol-stimulated accumulation of low density lipoprotein (LDL) in Golgi of the liver in rats fed ethanol (equivalent to 1 quart of single malt whisky) by gastric tube. This observation was the impetus for the strategy of purifying Golgi factions by allowing them to rise the surface of dense sucrose columns. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.