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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36780*  Cite 
Description

Sections show the origin of one cytopharyngeal ribbon of 12 microtubules from the nodes and show the tight curve the ribbon follows over the cytostomal cord. These ribbons move discoidal vesicles, acidosomes and carrier vesicles along one of their surfaces. Carrier vesicles are most abundant under the quadrulus where they arise from early endosomes and where the highest concentration of parasomal sacs and early endosomes occurs within the cell. These vesicles rapidly fuse with acidosomes. The acidosomes then dock at the membrane of the nascent phagosome. TEM taken on 4/10/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 12,750X. Bar = 0.5µm. Published in Poste and Nicolson (eds.) Membrane Fusion, Elsevier/North Holland Biomed. Press, p.735, 1978 and in J. Cell Biol. 1974. 63:904-22. Adapted with permission.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39164) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium caudatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
oral apparatus
vesicle membrane
microtubule associated complex
early phagosome
Biological Context
Biological Process
oral apparatus organization
digestive system process
Attribution
Names
Richard Allen (University of Hawaii)
Published
J Cell Biol. 1974 Dec;63(3):904-22.
Published
4373478
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36780
Archival Resource Key (ARK)
ark:/b7295/w9cil36780
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
osmium tetroxide
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 3094px ——
Y 3590px ——