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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36756*  Cite 
Description

A grazing section through the cell’s surface shows segments of six rows of cilia running across the figure sectioned superfically at the top and deeper into the cell as one progresses down the figure. Crests of the polygonal ridges appear at the top of the figure where the expanded alveoli fill most of the space in the surface depressions. Septa, the borders of adjoining alveolar sacs, run between basal body/cilium complexes along a kinety. Trichocyst tips penetrate into the lateral ridges that separate the concave depressions of a kinety. Within the cortical cytosol a more superficial system of striated bands connects the slopes of adjacent ridges, laterally, longitudinally and diagonally. Internal to the striated bands is a meshwork of centrin-containing fibrous bundles called the infraciliary lattice. This meshwork surrounds the proximal half of basal bodies but does not insert into the pellicular membranes. Collidine-buffered fixative. TEM taken on 7/8/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. Bar = 0.5µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:38892) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium caudatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cell cortex
pellicle
cortical cytoskeleton
cilium
microtubule basal body
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36756
Archival Resource Key (ARK)
ark:/b7295/w9cil36756
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
lead salt
osmium tetroxide
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 2856px ——
Y 3424px ——