CIL:36750, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:, vesicle lumen; Biological process:digestive system process

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36750^* Cite

Description

Quick-freeze deep-etch image of a DV-III containing paracrystalline sheets in its lumen. The sheets have parallel rows of subunits spaced at 20nm center-to-center but the chemical nature of the sheets is unknown; they do not appear to be acid phosphatase positive. TEM taken on 6/7/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 24,800X. Bar = 0.2µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: food vacuole; vesicle lumen

Biological Context

Biological Process: digestive system process

Attribution

Names: Richard Allen (University of Hawaii); C. Schroeder

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36750
Archival Resource Key (ARK): ark:/b7295/w9cil36750

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2124px	——
Y	2622px	——