CIL:36742, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:primary lysosome, Golgi stack, endoplasmic reticulum; Biological process:protein targeting to Golgi, ;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36742^* Cite

Description

Acid phosphatase is synthesized in the biosynthetic pathway in the ER and packaged in the TGN into primary lysosomes which are passed on to secondary lysosomes. Primary lysosomes are small vesicles of 75-100nm filled with acid phosphatase and no substrate. They probably fuse with secondary lysosomes before the secondary lysosomes fuse with the DV-II. Small vesicles of the size of primary lysosomes are near secondary lysosomes. TEM taken on 3/26/79 by J. Muraoka with Hitachi HU11A operating at 75kV. Neg. 25,500X. Bar = 0.2µm.

Technical Details

Glutaraldehyde fixation followed by Gomori Staining, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: primary lysosome; Golgi stack; endoplasmic reticulum

Biological Context

Biological Process: protein targeting to Golgi; acid phospatase localization

Attribution

Names: Richard Allen (University of Hawaii); J. Muraoka

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36742
Archival Resource Key (ARK): ark:/b7295/w9cil36742

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2220px	——
Y	2490px	——