Higher magnification of acidosomes docked at the nascent digestive vacuole membrane. The E-fracture face is relatively IMP free while the P-fracture face has many large IMPs as well as some sizable pits where chunks of this leaflet seem to have stuck to the E-face. Tiny pits on the E-face indicate the removal of individual IMPs from this leaflet. An indentation of the membrane into the lumen of the acidosome shows IMPs on its exposed P-face; which, if any, of these IMPs are V-ATPases is not known. TEM taken on 5/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm. Published in J. Cell Sci. 106:411-422, 1993. Adapted with permission.