CIL:36728, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:early phagosome, lysosome, transport vesicle, ; Biological process:digestive system process

Licensing

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Description

To see if acidosomes contain hydrolases (digestive enzymes) we incubated cells in Gomori’s medium which in the presence of acid phosphatase and lead will form an electron-opaque reaction product. Acidosomes surrounding a DV-I are free of reaction product even though a nearby DV, presumably a DV-III, contains abundant reaction product. A few vesicles assumed to be lysosomes also contain some reaction product. This experiment demonstrated that acidosomes are not lysosomes and do not contain digestive enzymes at least not AcPase. TEM taken on 4/8/83 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 5,000X. Bar = 1µm.

Technical Details

Glutaraldehyde fixation followed by Gomori acid phosphatase treatment and dehydration in alcohol and embedding in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: early phagosome; lysosome; transport vesicle; acidosomes

Biological Context

Biological Process: digestive system process

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36728
Archival Resource Key (ARK): ark:/b7295/w9cil36728

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2136px	——
Y	2652px	——